Anti-Toll-interacting protein Tollip Antibody Picoband®

About this product

Boster Bio Anti-Toll-interacting protein Tollip Antibody catalog # PA2005. Tested in Flow Cytometry, IP, ICC, IHC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2005

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

TOLLIP (TOLL-Interacting Protein), is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al. (2009) stated that the TOLLIP gene maps to chromosome 11. The mouse gene maps to chromosome 7. By Western blot analysis of embryonic kidney cells, Burns et al. (2000) confirmed the binding of TOLLIP to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al. (2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by TOLLIP-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of TOLLIP from IRAK, which can then transmit the IL1-induced signals.

Synonyms

Toll-interacting protein; TOLLIP

Gene Name

TOLLIP

Gene ID

54472

UniProt

Q9H0E2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Tollip.

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.

Applications

Flow Cytometry, IP, IF, IHC, ICC, WB

Field of Research

Cytokines, Immunoglobulins, Immunology, Innate Immunity, Interleukins, Receptors, TLR Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity (PubMed:11751856) . Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin- ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851) . .

References & Citations

1. Bulut, Y., Faure, E., Thomas, L., Equils, O., Arditi, M. Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 signaling. J. Immun. 167: 987-994, 2001. 2. Burns, K., Clatworthy, J., Martin, L., Martinon, F., Plumpton, C., Maschera, B., Lewis, A., Ray, K., Tschopp, J., Volpe, F. Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor. Nature Cell Biol. 2: 346-351, 2000. 3. Lo, Y.-L. S., Beckhouse, A. G., Boulus, S. L., Wells, C. A. Diversification of TOLLIP isoforms in mouse and man. Mammalian Genome 20: 305-314, 2009.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30282 MW

Observed Molecular Weight

30 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Toll-interacting protein

Subcellular Location

Cytoplasm .

Sequence Similarities

Belongs to the tollip family.

Protein Name

Toll-interacting protein

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.