Anti-TRAF1 Antibody Picoband®

About this product

Boster Bio Anti-TRAF1 Antibody catalog # PA2006. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2006

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

TRAF1 (TNF Receptor-Associated Factor 1), also called EBI6, is a protein that in humans is encoded by the TRAF1 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. Siemienski et al. (1997) used fluorescence in situ hybridization to map the TRAF1 gene to 9q33-q34. Mosialos et al. (1995) found that LMP1, the EBV-transforming protein, specifically associates with LAP1 (TRAF3) or EBI6 in B lymphoblasts. LMP1 expression res LAP1 and EBI6 from scattered cytoplasmic structures to LMP1 plasma membrane patches. Both LAP1 and EBI6 associated with the cytoplasmic domain of p80/TNFR2 in vivo.

Synonyms

TNF receptor-associated factor 1; Epstein-Barr virus-induced protein 6; TRAF1; EBI6

Gene Name

TRAF1

Gene ID

7185

UniProt

Q13077

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TRAF1, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.

Applications

WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Growth Factors, Growth Factors/Hormones, Host-Virus Interaction, Interspecies Interaction, Kinases/Phosphatases, Microbiology, NFKB Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Adapter molecule that regulates the activation of NF- kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein- ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. .

References & Citations

1. Mosialos, G., Birkenbach, M., Yalamanchili, R., VanArsdale, T., Ware, C., Kieff, E. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 80: 389-399, 1995. 2. Siemienski, K., Peters, N., Scheurich, P., Wajant, H. Organization of the human tumour necrosis factor receptor-associated factor 1 (TRAF1) gene and mapping to chromosome 9q33-34. Gene 195: 35-39, 1997. 3. Tsitsikov, E. N., Laouini, D., Dunn, I. F., Sannikova, T. Y., Davidson, L., Alt, F. W., Geha, R. S. TRAF1 is a negative regulator of TNF signaling: enhanced TNF signaling in TRAF1-deficient mice. Immunity 15: 647-657, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46164 MW

Observed Molecular Weight

46 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

TNF receptor-associated factor 1

Subcellular Location

Cytoplasm .

Sequence Similarities

Contains 1 MATH domain.

Protein Name

TNF receptor-associated factor 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.