Anti-Periostin/POSTN Antibody Picoband®

About this product

Boster Bio Anti-Periostin/POSTN Antibody catalog # PA2048. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2048

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

POSTN (Periostin), also known as PN or OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Gillan et al. (2002) found that purified recombinant PN supported adhesion of ovarian epithelial cells. Shao et al. (2004) found that periostin was overexpressed by the majority of human primary breast cancers examined. Transfected tumor cell lines overexpressing periostin showed accelerated growth and angiogenesis as xenografts in immunocompromised animals. Kuhn et al. (2007) showed that extracellular periostin induced reentry of differentiated mammalian cardiomyocytes into the cell cycle.

Synonyms

Periostin; PN; Osteoblast-specific factor 2; OSF-2; Postn; Osf2

Gene Name

POSTN

Gene ID

50706

UniProt

Q62009

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Periostin, identical to the related rat sequence, and different from the related human sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .

Applications

WB

Field of Research

Atherosclerosis, Cancer, Cardiogenesis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Structures, Tags & Cell Markers, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. .

References & Citations

1. Gillan, L., Matei, D., Fishman, D. A., Gerbin, C. S., Karlan, B. Y., Chang, D. D. Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha-V-beta-3 and alpha-V-beta-5 integrins and promotes cell motility. Cancer Res. 62: 5358-5364, 2002. 2. Kuhn, B., del Monte, F., Hajjar, R. J., Chang, Y.-S., Lebeche, D., Arab, S., Keating, M. T. Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair. Nature Med. 13: 962-969, 2007. 3. Shao, R., Bao, S., Bai, X., Blanchette, C., Anderson, R. M., Dang, T., Gishizky, M. L., Marks, J. R., Wang, X.-F. Acquired expression of periostin by human breast cancers promotes tumor angiogenesis through up-regulation of vascular endothelial growth factor receptor 2 expression. Molec. Cell. Biol. 24: 3992-4003, 2004.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

93144 MW

Observed Molecular Weight

93 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Periostin

Subcellular Location

Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi.

Sequence Similarities

Contains 1 EMI domain.

Protein Name

Periostin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.