Anti-Proprotein convertase PC4/PCSK4 Antibody Picoband®

About this product

Boster Bio Anti-Proprotein convertase PC4/PCSK4 Antibody catalog # PA2086. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2086

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PCSK4 (Proprotein Convertase, Subtilisin/Kexin-Type, 4), is an enzyme that in humans is encoded by the PCSK4 gene. The gene for PC4 (Pcsk4 locus) was mapped to mouse chromosome 10, close to the adipsin locus and near the gene encoding anti-mullerian hormone. Qiu et al. (2005) found that aberrant processing of pro-IGF2 by PC4 may be a cause of IUGR. PC4 cleaved pro-IGF2 to generate the intermediate processed form, IGF2 (1-102) . Mature IGF2 (1-67), likely resulting from further processing by carboxypeptidases, is capable of activating invasive trophoblast cells through AKT phosphorylation. Inhibition of PC4 by a PC4-specific inhibitor blocked pro-IGF2 processing and reduced trophoblast cell migration.

Synonyms

Proprotein convertase subtilisin/kexin type 4;3.4.21.-; KEX2-like endoprotease 3; Neuroendocrine convertase 3; NEC 3; Prohormone convertase 3; Pcsk4; Nec-3, Nec3

Gene Name

PCSK4

Gene ID

18551

UniProt

P29121

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4, different from the related rat sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed abundantly in the testis. .

Applications

WB

Field of Research

Cell Biology, Proteolysis/Ubiquitin, Proteolytic Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues.

References & Citations

1. Cao, H., Mok, A., Miskie, B., Hegele, R. A. Single-nucleotide polymorphisms of the proprotein convertase subtilisin/kexin type 5 (PCSK5) gene. J. Hum. Genet. 46: 730-732, 2001. 2. Mbikay, M., Seidah, N. G., Chretien, M., Simpson, E. M. Chromosomal assignment of the genes for proprotein convertases PC4, PC5, and PACE 4 in mouse and human. Genomics 26: 123-129, 1995. 3. Qiu, Q., Basak, A., Mbikay, M., Tsang, B. K., Gruslin, A. Role of pro-IGF-II processing by proprotein convertase 4 in human placental development. Proc. Nat. Acad. Sci. 102: 11047-11052, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

73214 MW

Observed Molecular Weight

83 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Proprotein convertase subtilisin/kexin type 4

Subcellular Location

Cytoplasmic vesicle, secretory vesicle. Localized in the secretion granules.

Sequence Similarities

Belongs to the peptidase S8 family. Furin subfamily.

Protein Name

Proprotein convertase subtilisin/kexin type 4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.