Anti-Aquaporin 10/AQP10 Antibody Picoband®

About this product

Boster Bio Anti-Aquaporin 10/AQP10 Antibody catalog # PA2095. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2095

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

AQP10 was identified in human small intestine. This gene encoded a 264-amino-acid protein with high sequence identity with AQP3 (53%), 9 (52%), and 7 (43%) . These AQPs constitute one subfamily of AQP family that is differentiated from the other subfamily of AQP (AQP0, 1, 2, 4, 5, 6, and 8) by sequence homology. Northern blot analysis revealed expression of a 2.3-kb AQP10 transcript in jejunum but not liver. RNase protection analysis detected nearly exclusive expression in duodenum and jejunum. In situ hybridization analysis demonstrated highest expression of AQP10 in absorptive epithelial cells at the tips of villi in the jejunum. SDS-PAGE analysis showed expression of a 28-kD AQP10 protein, similar to its predicted size as well as to that of the other AQPs. Functional analysis indicated that cells expressing AQP10 are permeable to relatively low amounts of water, but, unlike AQP3, AQP7, and AQP9, are not permeable to urea or glycerol.

Synonyms

Aquaporin-10; AQP-10; Aquaglyceroporin-10; Small intestine aquaporin; AQP10

Gene Name

AQP10

Gene ID

89872

UniProt

Q96PS8

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 10.

Clonality

Polyclonal

Tissue Specificity

Expressed exclusively in duodenum and jejunum. Highest expression in absorptive epithelial cells at the tips of villi in the jejunum.

Applications

WB

Field of Research

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Water channel required to promote glycerol permeability and water transport across cell membranes. May contribute to water transport in the upper portion of small intestine. Isoform 2 is not permeable to urea and glycerol. .

References & Citations

1. Hatakeyama, S., Yoshida, Y., Tani, T., Koyama, Y., Nihei, K., Ohshiro, K., Kamiie, J.-I., Yaoita, E., Suda, T., Hatakeyama, K., Yamamoto, T. Cloning of a new aquaporin (AQP10) abundantly expressed in duodenum and jejunum. Biochem. Biophys. Res. Commun. 287: 814-819, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31763 MW

Observed Molecular Weight

31 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Aquaporin-10

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Name

Aquaporin-10

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.