Anti-SMAD5 Antibody Picoband®

About this product

Boster Bio Anti-SMAD5 Antibody catalog # PA2115. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2115

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's) . It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.

Synonyms

Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5; MADH5

Gene Name

SMAD5

Gene ID

4090

UniProt

Q99717

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SMAD5, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

IHC, WB

Field of Research

Cytoplasmic, Developmental Biology, Epigenetics and Nuclear Signaling, Nuclear Signaling, Nuclear Signaling Pathways, Organogenesis, Signal Transduction, Signaling Pathway, Stem Cells, TGF Beta

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) .

References & Citations

1. Bruno, E., Horrigan, S. K., Van Den Berg, D., Rozler, E., Fitting, P. R., Moss, S. T., Westbrook, C., Hoffman, R. The Smad5 gene is involved in the intracellular signaling pathways that mediate the inhibitory effects of transforming growth factor-beta on human hematopoiesis. Blood 91: 1917-1923, 1998. 2. Davis, B. N., Hilyard, A. C., Lagna, G., Hata, A. SMAD proteins control DROSHA-mediated microRNA maturation. Nature 454: 56-61, 2008. 3. Gemma, A., Hagiwara, K., Vincent, F., Ke, Y., Hancock, A. R., Nagashima, M., Bennett, W. P., Harris, C. C. hSmad5 gene, a human hSmad family member: its full length cDNA, genomic structure, promoter region and mutation analysis in human tumors. Oncogene 16: 951-956, 1998. 4. Riggins, G. J., Thiagalingam, S., Rozenblum, E., Weinstein, C. L., Kern, S. E., Hamilton, S. R., Willson, J. K. V., Markowitz, S. D., Kinzler, K. W., Vogelstein, B. Mad-related genes in the human. Nature Genet. 13: 347-349, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

52258 MW

Observed Molecular Weight

52 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Mothers against decapentaplegic homolog 5

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

Sequence Similarities

Belongs to the dwarfin/SMAD family.

Protein Name

Mothers against decapentaplegic homolog 5

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.