Anti-Matrix metalloproteinase-14 MMP14 Antibody Picoband®

About this product

Boster Bio Anti-Matrix metalloproteinase-14 MMP14 Antibody catalog # PA2147. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2147

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Matrix metalloproteinase-14, also called MT-MMP 1, is an enzyme that in humans is encoded by the MMP14 gene. The protein encoded by this gene is a member of the membrane-type MMP subfamily. This gene is mapped to 14q11.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene may be involved in actin cytoskeleton reorganization by cleaving PTK7. It acts as a positive regulator of cell growth and migration via activation of MMP15.

Synonyms

Matrix metalloproteinase-14; MMP-14;3.4.24.80; MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP; MMP14

Gene Name

MMP14

Gene ID

4323

UniProt

P50281

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MMP14, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. .

Applications

IHC, WB

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metabolism, Metabolism Processes, Metalloprotease, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Response To Hypoxia, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro- MMP2. .

References & Citations

1. Chun, T.-H., Hotary, K. B., Sabeh, F., Saltiel, A. R., Allen, E. D., Weiss, S. J. A pericellular collagenase s the 3-dimensional development of white adipose tissue. Cell 125: 577-591, 2006. 2. Oh, J., Takahashi, R., Adachi, E., Kondo, S., Kuratomi, S., Noma, A., Alexander, D. B., Motoda, H., Okada, A., Seiki, M., Itoh, T., Itohara, S., Takahashi, C., Noda, M. Mutations in two matrix metalloproteinase genes, MMP-2, and MT1-MMP, are synthetic lethal in mice. Oncogene 23: 5041-5048, 2004. 3. Ueda, M., Yamashita, Y., Takehara, M., Terai, Y., Kumagai, K., Ueki, K., Kanda, K., Yamaguchi, H., Akise, D., Hung, Y.-C., Ueki, M. Survivin gene expression in endometriosis. J. Clin. Endocr. Metab. 87: 3452-3459, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

65894 MW

Observed Molecular Weight

150 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Matrix metalloproteinase-14

Subcellular Location

Membrane ; Single-pass type I membrane protein . Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.

Sequence Similarities

Belongs to the peptidase M10A family.

Protein Name

Matrix metalloproteinase-14

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.