Anti-Cyclophilin B/PPIB Antibody Picoband®

About this product

Boster Bio Anti-Cyclophilin B/PPIB Antibody catalog # PA2151. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2151

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Peptidyl-prolyl cis-trans isomerase B, also known as CYPB, is an enzyme that in humans is encoded by the PPIB gene. This gene is mapped to 15q22.31. The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.

Synonyms

Peptidyl-prolyl cis-trans isomerase B; PPIase B;5.2.1.8; CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP; PPIB; CYPB

Gene Name

PPIB

Gene ID

5479

UniProt

P23284

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cyclophilin B, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Clonality

Polyclonal

Applications

IHC, WB

Field of Research

ER Proteins, Immunology, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

References & Citations

1. Barnes, A. M., Cabral, W. A., Weis, M., Makareeva, E., Mertz, E. L., Leikin, S., Eyre, D., Trujillo, C., Marini, J. C. Absence of FKBP10 in recessive type XI osteogenesis imperfecta leads to diminished collagen cross-linking and reduced collagen deposition in extracellular matrix. Hum. Mutat. 33: 1589-1598, 2012. 2. van Dijk, F. S., Cobben, J. M., Pals, G. Osteogenesis imperfecta, normal collagen folding, and lack of cyclophilin B. (Letter) New Eng. J. Med. 362: 1940-1941, 2010. 3. Watashi, K., Ishii, N., Hijikata, M., Inoue, D., Murata, T., Miyanari, Y., Shimotohno, K.Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase.Molec. Cell 19: 111-122, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23743 MW

Observed Molecular Weight

18 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Peptidyl-prolyl cis-trans isomerase B

Subcellular Location

Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Sequence Similarities

Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.

Protein Name

Peptidyl-prolyl cis-trans isomerase B

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.