Anti-Profilin 2/PFN2 Antibody Picoband®

About this product

Boster Bio Anti-Profilin 2/PFN2 Antibody catalog # PA2162. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2162

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Profilin-2, also called PFN2, is a protein that in humans is encoded by the PFN2 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. This gene is mapped to 3q25.1. It is though to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. It binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Synonyms

Profilin-2; Profilin II; PFN2

Gene Name

PFN2

Gene ID

5217

UniProt

P35080

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 2, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.

Applications

IF, IHC, WB

Field of Research

Actin, Actin Assembly, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

References & Citations

1. Gieselmann, R., Kwiatkowski, D. J., Janmey, P. A., Witke, W. Distinct biochemical characteristics of the two human profilin isoforms. Europ. J. Biochem. 229: 621-628, 1995. 2. Joensuu, T., Blanco, G., Pakarinen, L., Sistonen, P., Kaariainen, H., Brown, S., de la Chapelle, A., Sankila, E.-M. Refined mapping of the Usher syndrome type III locus on chromosome 3, exclusion of candidate genes, and identification of the putative mouse homologous region. Genomics 38: 255-263, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

15 kDa

Observed Molecular Weight

15 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Profilin-2

Subcellular Location

Cytoplasm, cytoskeleton.

Sequence Similarities

Contains 17 laminin EGF-like domains.

Protein Name

Profilin-2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.