Anti-SGLT1/SLC5A1 Antibody Picoband®

About this product

Boster Bio Anti-SGLT1/SLC5A1 Antibody catalog # PA2244. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2244

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sodium/glucose cotransporter 1 (SLC5A1), also known as NAGT or SGLT1 is a protein that in humans is encoded by the SLC5A1 gene. This gene is mapped to 22q12.3. This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Sodium/glucose cotransporter 1; Na (+) /glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1; SLC5A1; NAGT, SGLT1

Gene Name

SLC5A1

Gene ID

6523

UniProt

P13866

Host

Rabbit

Reactivity

Rat, Mouse, Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse SGLT1, different from the related rat sequence by one amino acid, and from the related human sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed mainly in intestine and kidney.

Applications

WB

Field of Research

Channels, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Actively transports glucose into cells by Na (+) cotransport with a Na (+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na (+) /glucose cotransporter arranged in series along kidney proximal tubules.

References & Citations

1. Margolskee, R. F., Dyer, J., Kokrashvili, Z., Salmon, K. S. H., Ilegems, E., Daly, K., Maillet, E. L., Ninomiya, Y., Mosinger, B., Shirazi-Beechey, S. P. T1R3 and gustducin in gut sense sugars to regulate expression of Na (+) -glucose cotransporter 1. Proc. Nat. Acad. Sci. 104: 15075-15080, 2007. 2. Turk, E, Wright, E. M. Membrane topology motifs in the SGLT cotransporter family. J. Membr. Biol. 159: 1-20, 1997. 3. Wright, E. M., Turk, E., Zabel, B., Mundlos, S., Dyer, J. Molecular genetics of intestinal glucose transport. J. Clin. Invest. 88: 1435-1440, 1991.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

73498 MW

Observed Molecular Weight

73 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Sodium/glucose cotransporter 1

Subcellular Location

Membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Protein Name

Sodium/glucose cotransporter 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.