Anti-PON1 Antibody Picoband®

About this product

Boster Bio Anti-PON1 Antibody catalog # PA2253. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2253

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serum paraoxonase/arylesterase 1 (PON1), also known as aromatic esterase 1, is an enzyme that in humans is encoded by the PON1 gene. It is mapped to 7q21.3. This gene has  esterase  and more specifically  paraoxonase activity. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL) . The PON1 gene is activated by PPAR-gamma, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis.

Synonyms

Serum paraoxonase/arylesterase 1; PON 1;3.1.1.2;3.1.1.81;3.1.8.1; Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1; PON1; PON

Gene Name

PON1

Gene ID

5444

UniProt

P27169

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PON1, different from the related mouse and rat sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Plasma, associated with HDL (at protein level) . Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. .

Applications

WB

Field of Research

Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. .

References & Citations

1. Costa LG, Cole TB, Vitalone A, Furlong CE (2005) . Measurement of paraoxonase (PON1) status as a potential biomarker of susceptibility to organophosphate toxicity. CLINICA CHEMICA ACTA 352 (1-2) : 37–47. 2. Khateeb J, Gantman A, Kreitenberg AJ, Aviram M, Fuhrman B (January 2010) . Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-gamma pathway.Atherosclerosis 208 (1) : 119–25. 3. Primo-Parmo SL, Sorenson RC, Teiber J, La Du BN (May 1996) . The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family. Genomics 33 (3) : 498–507.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39731 MW

Observed Molecular Weight

40 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Serum paraoxonase/arylesterase 1

Subcellular Location

Secreted, extracellular space.

Sequence Similarities

Belongs to the paraoxonase family.

Protein Name

Serum paraoxonase/arylesterase 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.