Anti-Plakophilin 2/PKP2 Antibody Picoband®

About this product

Boster Bio Anti-Plakophilin 2/PKP2 Antibody catalog # PA2278. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2278

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Plakophilin-2 is a protein that in humans is encoded by the PKP2 gene. This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. It is mapped to 12p11.21. PKP2 is a constituent of the desmosomal plaque in simple epithelia, some stratified epithelia, and some nonepithelial cells. PKP2 is also enriched in the karyoplasm of cells of various types, including those lacking desmosomes. Plakophilin proteins participate in linking cadherins to intermediate filaments in the cytoskeleton and this gene product may regulate the signaling activity of beta-catenin. PKP2 has been shown to interact with Desmoplakin, Plakoglobin and Desmoglein 1.

Synonyms

Plakophilin-2; PKP2

Gene Name

PKP2

Gene ID

5318

UniProt

Q99959

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Plakophilin 2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Detected in heart right ventricle (at protein level) . Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata. .

Applications

WB

Field of Research

Angiogenesis, Cardiovascular, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Neuroscience, Signal Transduction, Structures

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May play a role in junctional plaques. .

References & Citations

1 Entrez Gene: PKP2 plakophilin 2 2 Marcus, F. I., Fontaine, G. H., Guiraudon, G., Frank, R., Laurenceau, J. L., Malergue, C., Grosgogeat, Y. Right ventricular dysplasia: a report of 24 adult cases. Circulation 65: 384-398, 1982. 3 Mertens C, Kuhn C, Franke WW (Jan 1997) . Plakophilins 2a and 2b: constitutive proteins of dual location in the karyoplasm and the desmosomal plaque. J Cell Biol 135 (4) : 1009–25.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

97415 MW

Observed Molecular Weight

97 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Plakophilin-2

Subcellular Location

Nucleus . Cell junction, desmosome . Nuclear and associated with desmosomes.

Sequence Similarities

Belongs to the beta-catenin family.

Protein Name

Plakophilin-2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.