Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband®

About this product

Boster Bio Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband® catalog # PB9002. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9002

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1) or mitochondrial cytochrome c oxidase subunit 1, is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, it was demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

Synonyms

Prostaglandin G/H synthase 1;1.14.99.1; Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1; PTGS1; COX1

Gene Name

PTGS1

Gene ID

5742

UniProt

P23219

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human COX1 recombinant protein (Position: E318-L599) . Human COX1 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat COX1, respectively.

Clonality

Polyclonal

Tissue Specificity

Synthesized exclusively by adipocytes and secreted into plasma.

Applications

Flow Cytometry, IHC, WB

Field of Research

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Endocrine Metabolism, Heart Disease, Hormone Biosynthesis, Immunology, Innate Immunity, Lipid and Lipoprotein Metabolism, Lipid Signaling, Macrophage/Inflammation, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Oxidative Stress, Pathways and Processes, Platelets, Redox Metabolism, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.

References & Citations

1. Yokoyama, C.; Tanabe, T. : Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme. Biochem. Biophys. Res. Commun. 165: 888-894, 1989. 2. Langenbach, R.; Morham, S. G.; Tiano, H. F.; Loftin, C. D.; Ghanayem, B. I.; Chulada, P. C.; Mahler, J. F.; Lee, C. A.; Goulding, E. H.; Kluckman, K. D.; Kim, H. S.; Smithies, O. : Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration. Cell 83: 483-492, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68686 MW

Observed Molecular Weight

65-72 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Prostaglandin G/H synthase 1

Subcellular Location

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Sequence Similarities

Belongs to the prostaglandin G/H synthase family.

Protein Name

Prostaglandin G/H synthase 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.