Anti-MMP7 Antibody Picoband®

About this product

Boster Bio Anti-MMP7 Antibody Picoband® catalog # PB9038. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9038

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The MMP-7 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The MMP-7 protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively) . Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. MMP-7, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, MMP-7 mRNA is regulated in a specific and distinct manner in normal and malignant tissues. MMP-7 has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined. MMP-7 is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.

Synonyms

Matrilysin;3.4.24.23; Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase; Mmp7

Gene Name

MMP7

Gene ID

17393

UniProt

Q10738

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived mouse MMP7 recombinant protein (Position: Y95-L264) . Mouse MMP7 shares 71% and 90% amino acid (aa) sequences identity with human and rat MMP7, respectively.

Clonality

Polyclonal

Tissue Specificity

Widely distributed in various tissues.

Applications

ELISA, IHC, WB

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase (By similarity) . .

References & Citations

1. Masaki, T.; Matsuoka, H.; Sugiyama, M.; Abe, N.; Goto, A.; Sakamoto, A.; Atomi, Y. Matrilysin (MMP-7) as a significant determinant of malignant potential of early invasive colorectal carcinomas. Brit. J. Cancer 84: 1317-1321, 2001. 2. Gaire, M.; Magbanua, Z.; McDonnell, S.; McNeil, L.; Lovett, D. H.; Matrisian, L. M. Structure and expression of the human gene for the matrix metalloproteinase matrilysin. J. Biol. Chem. 269: 2032-2040, 1994. 3. Zuo, F.; Kaminski, N.; Eugui, E.; Allard, J.; Yakhini, Z.; Ben-Dor, A.; Lollini, L.; Morris, D.; Kim, Y.; DeLustro, B.; Sheppard, D.; Pardo, A.; Selman, M.; Heller, R. A. Gene expression analysis reveals matrilysin as a key regulator of pulmonary fibrosis in mice and humans. Proc. Nat. Acad. Sci. 99: 6292-6297, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29755 MW

Observed Molecular Weight

30 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Matrilysin

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the peptidase M10A family.

Protein Name

Matrilysin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.