Anti-Annexin V/ANXA5 Antibody Picoband®

About this product

Boster Bio Anti-Annexin V/ANXA5 Antibody Picoband® catalog # PB9044. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9044

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an in inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Synonyms

Annexin A5; Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; PP4; Placental anticoagulant protein I; PAP-I; Thromboplastin inhibitor; Vascular anticoagulant-alpha; VAC-alpha; ANXA5; ANX5, ENX2, PP4

Gene Name

ANXA5

Gene ID

308

UniProt

P08758

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Annexin V recombinant protein (Position: A2-D320) . Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in all cell types so far analyzed.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Apoptosis, Apoptotic Markers, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Invasion/Microenvironment

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This protein is an anticoagulant protein that acts as an in inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

References & Citations

1. Bogdanova, N., Horst, J., Chlystun, M., Croucher, P. J. P., Nebel, A., Bohring, A., Todorova, A., Schreiber, S., Gerke, V., Krawczak, M., Markoff, A. A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy loss.Hum. Molec. Genet. 16: 573-578, 2007. 2. Demange, P., Voges, D., Benz, J., Liemann, S., Gottig, P., Berendes, R., Burger, A., Huber, R. Annexin V: the key to understanding ion selectivity and voltage regulation?Trends Biochem. Sci. 19: 272-276, 1994. 3. Sofiadis A, Becker S, Hellman U, Hultin-Rosenberg L, Dinets A, Hulchiy M, Zedenius J, Wallin G, Foukakis T, Höög A, Auer G, Lehtiö J, Larsson C. (April 2012) . Proteomic profiling of follicular and papillary thyroid tumors.. Eur J Endocrinol.. 166 (4) : 657–67.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36 kDa

Observed Molecular Weight

36 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Annexin A5

Subcellular Location

Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of the plasma membrane containing increased PI (3,4,5) P3 or PI (3,4) P2, cell membrane targeting is also facilitared by interaction with CLIP3.

Sequence Similarities

Belongs to the annexin family.

Protein Name

Annexin A5

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.