- Catalog number
- PB9047
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
- Synonyms
- Cathepsin D;3.4.23.5; Cathepsin D light chain; Cathepsin D heavy chain; CTSD; CPSD
- Gene Name
- CTSD
- Gene ID
- 1509
- UniProt
- P07339
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E.coli-derived human Cathepsin D recombinant protein (Position: G65-L412) . Human Cathepsin D shares 85% amino acid (aa) sequence identity with both mouse and rat Cathepsin D.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in the aorta extrcellular space (at protein level) . .
- Applications
- IHC, WB
- Field of Research
- Cancer, Cell Adhesion Proteins, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
- References & Citations
- 1. Faust PL, Kornfeld S, Chirgwin JM (Sep 1985) . Cloning and sequence analysis of cDNA for human cathepsin D. Proc Natl Acad Sci U S A 82 (15) : 4910–4. 2. Wolf M, Clark-Lewis I, Buri C, Langen H, Lis M, Mazzucchelli L (April 2003) . Cathepsin D specifically cleaves the chemokines macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, and SLC that are expressed in human breast cancer. Am. J. Pathol. 162 (4) : 1183–90.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 44552 MW
- Observed Molecular Weight
- 40 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Cathepsin D
- Subcellular Location
- Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380) . .
- Sequence Similarities
- Belongs to the peptidase A1 family.
- Protein Name
- Cathepsin D
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
