Anti-Cathepsin D/CTSD Antibody Picoband®

About this product

Boster Bio Anti-Cathepsin D/CTSD Antibody Picoband® catalog # PB9048. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9048

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Synonyms

Cathepsin D;3.4.23.5; Ctsd

Gene Name

CTSD

Gene ID

13033

UniProt

P18242

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410) . Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in the aorta extrcellular space (at protein level) . .

Applications

IF, IHC, ICC, WB

Field of Research

Cancer, Cell Adhesion Proteins, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acid protease active in intracellular protein breakdown.

References & Citations

1. Faust PL, Kornfeld S, Chirgwin JM (Sep 1985) . Cloning and sequence analysis of cDNA for human cathepsin D. Proc Natl Acad Sci U S A 82 (15) : 4910–4. 2. Wolf M, Clark-Lewis I, Buri C, Langen H, Lis M, Mazzucchelli L (April 2003) . Cathepsin D specifically cleaves the chemokines macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, and SLC that are expressed in human breast cancer. Am. J. Pathol. 162 (4) : 1183–90.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44954 MW

Observed Molecular Weight

45 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cathepsin D

Subcellular Location

Lysosome. Melanosome . Secreted, extracellular space .

Sequence Similarities

Belongs to the peptidase A1 family.

Protein Name

Cathepsin D

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.