Anti-Mast Cell Chymase/CMA1 Antibody Picoband®

About this product

Boster Bio Anti-Mast Cell Chymase/CMA1 Antibody Picoband® catalog # PB9055. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9055

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. Chymase is mapped to 14q12. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II.

Synonyms

Chymase;3.4.21.39; Alpha-chymase; Mast cell protease I; CMA1; CYH, CYM

Gene Name

CMA1

Gene ID

1215

UniProt

P23946

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CMA1 recombinant protein (Position: I22-N247) . Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively.

Clonality

Polyclonal

Tissue Specificity

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .

Applications

IHC, WB

Field of Research

Atherosclerosis, Cardiovascular, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

References & Citations

1. Caughey, GH. Mast cell tryptases and chymases in inflammation and host defense. Immu Revs 2007 (217) : 141-154. 2. Chmelar, J., Oliveira, C. J., Rezacova, P., Francischetti, I. M. B., Kovarova, Z., Pejler, G., Kopacek, P., Ribeiro, J. M. C., Mares, M., Kopecky, J., Kotsyfakis, M. A tick salivary protein targets cathepsin G and Chymase and inhibits host inflammation and platelet aggregation. Blood 117: 736-744, 2011. 3. Urata, H., Boehm, K. D., Philip, A., Kinoshita, A., Gabrovsek, J., Bumpus, F. M., Husain, A.Cellular localization and regional distribution of an angiotensin II-forming Chymase in the heart. J. Clin. Invest. 91: 1269-1281, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

27325 MW

Observed Molecular Weight

35 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Chymase

Subcellular Location

Secreted. Cytoplasmic granule. Mast cell granules.

Sequence Similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Protein Name

Chymase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.