Anti-Myeloperoxidase/MPO Antibody Picoband®

About this product

MPO Antibody

Specifications

Catalog number

PB9057

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.

Synonyms

Myeloperoxidase; MPO;1.11.2.2; Myeloperoxidase;89 kDa myeloperoxidase;84 kDa myeloperoxidase; Myeloperoxidase light chain; Myeloperoxidase heavy chain; MPO

Gene Name

MPO

Gene ID

4353

UniProt

P05164

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Myeloperoxidase recombinant protein (Position:S406-S745) . Human Myeloperoxidase shares 90% amino acid (aa) sequence identity with mouse Myeloperoxidase.

Clonality

Polyclonal

Tissue Specificity

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Pathways and Processes, Redox Metabolism, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

References & Citations

1. Klebanoff, S. J. : Myeloperoxidase. Proc. Assoc. Am. Phys. 111: 383-389, 1999. 2. Murao, S.-I.; Stevens, F. J.; Ito, A.; Huberman, E. : Myeloperoxidase: a myeloid cell nuclear antigen with DNA-binding properties. Proc. Nat. Acad. Sci. 85: 1232-1236, 1988. 3. Nauseef, W. M.; Olsson, I.; Arnljots, K. : Biosynthesis and processing of myeloperoxidase--a marker for myeloid cell differentiation. Europ. J. Haemat. 40: 97-110, 1988.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

83869 MW

Observed Molecular Weight

60-65 kDa, 80-90 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Myeloperoxidase

Subcellular Location

Lysosome.

Sequence Similarities

Belongs to the peroxidase family. XPO subfamily.

Protein Name

Myeloperoxidase

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.