Anti-Von Willebrand Factor/VWF Antibody Picoband®

About this product

Boster Bio Anti-Von Willebrand Factor/VWF Antibody Picoband® catalog # PB9062. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9062

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Synonyms

Von Willebrand factor; vWF; von Willebrand antigen 2; von Willebrand antigen II; VWF; F8VWF

Gene Name

VWF

Gene ID

7450

UniProt

P04275

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human VWF recombinant protein (Position: R2535-K2813) . Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF.

Clonality

Polyclonal

Tissue Specificity

Plasma.

Applications

Flow Cytometry, IHC, IHC-F, ICC, WB

Field of Research

Angiogenesis, Cancer, Cardiovascular, Invasion/Microenvironment, Platelets, Serum Proteins, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

References & Citations

1. Sadler JE (1998) . Biochemistry and genetics of von Willebrand factor. Annu. Rev. Biochem. 67: 395–424. 2. Ginsburg, D., Handin, R. I., Bonthron, D. T., Donlon, T. A., Bruns, G. A. P., Latt, S. A., Orkin, S. H. Human von Willebrand factor (VWF) : isolation of complementary DNA (cDNA) clones and chromosomal localization. Science 228: 1401-1406, 1985. 3. Sporn, L. A., Marder, V. J., Wagner, D. D. Von Willebrand factor released from Weibel-Palade bodies binds more avidly to extracellular matrix than that secreted constitutively. Blood 69: 1531-1534, 1987.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

309265 MW

Observed Molecular Weight

309 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Von Willebrand factor

Subcellular Location

Secreted . Secreted, extracellular space, extracellular matrix . Localized to storage granules.

Sequence Similarities

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Protein Name

Von Willebrand factor

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.