Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband®

About this product

Boster Bio Anti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband® catalog # PB9064. Tested in IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9064

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in human is encoded by the CCL4 gene. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize CCL4. Based on functional analysis of CCL4, one or more additional beta chemokine receptors that transduce CCL4 signals must also exist. MIP-1alpha and CCL4 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14) .

Synonyms

C-C motif chemokine 4; Macrophage inflammatory protein 1-beta; MIP-1-beta; Small-inducible cytokine A4; Ccl4; Mip1b, Scya4

Gene Name

CCL4

Gene ID

116637

UniProt

P50230

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived rat CCL4 recombinant protein (Position: A24-N92) . Rat CCL4 shares 80% and 86% amino acid (aa) sequences identity with human and mouse CCL4, respectively.

Clonality

Polyclonal

Tissue Specificity

Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level) . .

Applications

IHC, WB

Field of Research

Antiviral Signaling, Chemokines, Immune System Diseases, Immunology, Innate Immunity, Macrophage/Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Monokine with inflammatory and chemokinetic properties.

References & Citations

1. Irving SG, Zipfel PF, Balke J, McBride OW, Morton CC, Burd PR, Siebenlist U, Kelly K (June 1990) . Nucleic Acids Res. 18 (11) : 3261–70. 2. Bystry RS, Aluvihare V, Welch KA, Kallikourdis M, Betz AG (December 2001) . B cells and professional APCs recruit regulatory T cells via CCL4. Nat. Immunol. 2 (12) : 1126–32. 3. Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P (December 1995) . Science (journal) 270 (5243) : 1811–5. 4. Kamin-Lewis R, Abdelwahab SF, Trang C, Baker A, DeVico AL, Gallo RC, Lewis GK (July 2001) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10234 MW

Observed Molecular Weight

36 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

C-C motif chemokine 4

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the Wnt family.

Protein Name

C-C motif chemokine 4

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.