Anti-GDNF Antibody Picoband®

About this product

Boster Bio Anti-GDNF Antibody Picoband® catalog # PB9069. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9069

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF is also a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. In addition to its potential role in the differentiation and survival of central nervous system neurons, it has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.

Synonyms

Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor; ATF; GDNF

Gene Name

GDNF

Gene ID

2668

UniProt

P39905

Host

Rabbit

Reactivity

Rat, Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human GDNF recombinant protein (Position: S78-I211) . Human GDNF shares 93% amino acid (aa) sequence identity with both mouse and rat GDNF.

Clonality

Polyclonal

Tissue Specificity

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. .

Applications

WB

Field of Research

Developmental Biology, Growth and Development, Neurodegenerative Disease, Neurology Process, Neuroscience, Organogenesis

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

References & Citations

1. Lin, L.-F. H.; Doherty, D. H.; Lile, J. D.; Bektesh, S.; Collins, F. GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons. Science 260: 1130-1132, 1993. 2. Bermingham, N.; Hillermann, R.; Gilmour, F.; Martin, J. E.; Fisher, E. M. Human glial cell line-derived neurotrophic factor (GDNF) maps to chromosome 5. Hum. Genet. 96: 671-673, 1995. 3. Durbec, P.; Marcos-Gutierrez, C. V.; Kilkenny, C.; Grigoriou, M.; Wartiowaara, K.; Suvanto, P.; Smith, D.; Ponder, B.; Costantini, F.; Saarma, M.; Sariola, H.; Pachnis, V. GDNF signalling through the Ret receptor tyrosine kinase. Nature 381: 789-793, 1996.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23720 MW

Observed Molecular Weight

24 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Glial cell line-derived neurotrophic factor

Subcellular Location

Secreted .

Sequence Similarities

Belongs to the TGF-beta family. GDNF subfamily.

Protein Name

Glial cell line-derived neurotrophic factor

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.