Anti-5 Lipoxygenase/ALOX5 Antibody Picoband®

About this product

Boster Bio Anti-5 Lipoxygenase/ALOX5 Antibody catalog # RP1031. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1031

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.

Synonyms

Arachidonate 5-lipoxygenase;5-LO;5-lipoxygenase;1.13.11.34; ALOX5; LOG5

Gene Name

ALOX5

Gene ID

240

UniProt

P09917

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human ALOX5 recombinant protein (Position: A120-R483) . Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.

Clonality

Polyclonal

Applications

IHC, WB

Field of Research

Lipid and Lipoprotein Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. .

References & Citations

1. Luo, M., Jones, S. M., Peters-Golden, M., Brock, T. G. Nuclear localization of 5-lipoxygenase as a determinant of leukotriene B (4) synthetic capacity. Proc. Nat. Acad. Sci. 100: 12165-12170, 2003. 2. Chu, J., Pratico, D. 5-lipoxygenase as an endogenous modulator of amyloid-beta formation in vivo. Ann. Neurol. 69: 34-46, 2011.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

77983 MW

Observed Molecular Weight

78 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Arachidonate 5-lipoxygenase

Subcellular Location

Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Sequence Similarities

Belongs to the lipoxygenase family.

Protein Name

Arachidonate 5-lipoxygenase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.