Anti-Galectin 3/LGALS3 Antibody Picoband®

About this product

Boster Bio Anti-Galectin 3/LGALS3 Antibody Picoband® catalog # PB9081. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9081

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21– q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Synonyms

Galectin-3; Gal-3;35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3; MAC2

Gene Name

LGALS3

Gene ID

3958

UniProt

P17931

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250) . Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively.

Clonality

Polyclonal

Tissue Specificity

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity) . In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. .

References & Citations

1. Dumic J, Dabelic S, Flögel M. (2006) . Galectin-3: an open-ended story.. BBA General Subjects 1760 (4) : 616–635. 2. Liu F, Patterson RJ, Wang JL. (2002) . Intracellular function of galectins.. BBA General Subjects 1572 (2-3) : 263–273. 3. Cooper D. (2002) . Galectinomics: finding themes in complexity.. BBA General Subjects 1572 (2-3) : 209–231. 4. Raimond J, Zimonjic DB, Mignon C, et al. (1997) . Mapping of the galectin-3 gene (LGALS3) to human chromosome 14 at region 14q21—22.. Mamm. Genome 8 (9) : 706–707.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26152 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Galectin-3

Subcellular Location

Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface.

Sequence Similarities

Contains 1 galectin domain.

Protein Name

Galectin-3

Isotype

Rabbit IgG

Contents

243