Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody catalog # P00014-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Specifications

Catalog number

P00014-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

S1981

Synonyms

Serine-protein kinase ATM;2.7.11.1; Ataxia telangiectasia mutated; A-T mutated; ATM

Gene Name

ATM

Gene ID

472

UniProt

Q13315

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-ATM (S1981)

Clonality

Monoclonal

Clone

Clone: AEE-1

Tissue Specificity

Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

Applications

WB, IHC, ICC, IF, IP

Field of Research

Cancer, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Tumor Suppressors

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

350687 MW

Observed Molecular Weight

185 kDa

Gene Name Synonym

Serine-protein kinase ATM

Subcellular Location

Nucleus. Cytoplasmic vesicle. Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.