Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody catalog # P01388. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

P01388

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

Y817

Synonyms

BDNF/NT-3 growth factors receptor;2.7.10.1; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; NTRK2; TRKB

Gene Name

NTRK2

Gene ID

4915

UniProt

Q16620

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-TrkB (Y817)

Clonality

Monoclonal

Clone

Clone: EGA-14

Tissue Specificity

Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain. .

Applications

WB, IHC, ICC, IF, IP

Field of Research

Cancer, Growth and Development, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin- 4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin- dependent calcium signaling in glial cells and mediate communication between neurons and glia. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

91999 MW

Observed Molecular Weight

130 kDa

Gene Name Synonym

BDNF/NT-3 growth factors receptor

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endosome membrane ; Single-pass type I membrane protein . Internalized to endosomes upon ligand-binding. .

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.