Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-YAP1 (S127) Rabbit Monoclonal Antibody catalog # P00116. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

P00116

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

S127

Synonyms

Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP1; YAP65

Gene Name

YAP1

Gene ID

10413

UniProt

P46937

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human YAP1

Clonality

Monoclonal

Clone

Clone: GAB-25

Tissue Specificity

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level) . .

Applications

WB, IHC

Field of Research

Adapters, Cancer, Cancer Susceptibility, Cytoplasmic, Epigenetics and Nuclear Signaling, Protein Phosphorylation, Proto-Oncogenes, Signal Transduction, Transcription, Transcription Factors, Tyrosine Kinases

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637) . The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288) . Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702) . Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288) . The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

54462 MW

Observed Molecular Weight

70, 75 kDa

Gene Name Synonym

Transcriptional coactivator YAP1

Subcellular Location

Cytoplasm . Nucleus . Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001) . PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271) . .

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.