Anti-Phospho-EGFR (S695) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-EGFR (S695) Rabbit Monoclonal Antibody catalog # P00023-4. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Specifications

Catalog number

P00023-4

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

S695

Synonyms

Epidermal growth factor receptor;2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB, ERBB1, HER1

Gene Name

EGFR

Gene ID

1956

UniProt

P00533

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human EGFR

Clonality

Monoclonal

Clone

Clone: GCC-5

Tissue Specificity

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. .

Applications

WB, ICC, IF

Field of Research

Cancer, Growth Factors/Hormones, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tumor Biomarkers, Tyrosine Kinases

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also ly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

134277 MW

Observed Molecular Weight

185 kDa

Gene Name Synonym

Epidermal growth factor receptor

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) .

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.