Anti-Phospho-PP2A alpha (Y307) PPP2CA Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-PP2A alpha (Y307) PPP2CA Rabbit Monoclonal Antibody catalog # P01893. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

P01893

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

Y307

Synonyms

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha;3.1.3.16; Replication protein C; RP-C; PPP2CA

Gene Name

PPP2CA

Gene ID

5515

UniProt

P67775

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-PP2A alpha (Y307)

Clonality

Monoclonal

Clone

Clone: AAA-16

Tissue Specificity

Expressed in brain, and to a lesser extent in lung, kidney and testis.

Applications

WB, IHC, ICC, IF, IP

Field of Research

Cancer, Cardiovascular, Cell Biology, Cell Cycle, Contractility, Cytoplasmic, Epigenetics and Nuclear Signaling, Intracellular Signaling, Kinases/Phosphatases, Neuroscience, Neurotransmission, Protein Phosphorylation, Ser/Thr Phosphatases, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

PP2A is the major phosphatase for microtubule-associated proteins (MAPs) . PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity) . Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35594 MW

Observed Molecular Weight

266 kDa

Gene Name Synonym

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Subcellular Location

Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity) . .

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.