Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody catalog # P00059-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

P00059-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Phospho Site

S423, S425

Synonyms

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Gene Name

SMAD3

Gene ID

4088

UniProt

P84022

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human Phospho-Smad3 (S423 + S425)

Clonality

Monoclonal

Clone

Clone: AHO-19

Tissue Specificity

Widely expressed. Isoform 3 is not expressed in the pituitary gland. .

Applications

WB, IHC, ICC, IF

Field of Research

Cancer Metabolism, Cytoplasmic, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Pathways and Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, TGF Beta

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48081 MW

Observed Molecular Weight

90-100 kDa

Gene Name Synonym

Mothers against decapentaplegic homolog 3

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.