Anti-YY1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-YY1 Rabbit Monoclonal Antibody catalog # M00833. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00833

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Transcriptional repressor protein YY1; Delta transcription factor; INO80 complex subunit S; NF-E1; Yin and yang 1; YY-1; YY1; INO80S

Gene Name

YY1

Gene ID

7528

UniProt

P25490

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human YY1

Clonality

Monoclonal

Clone

Clone: AIE-25

Tissue Specificity

Most abundant in liver, brain and lung.

Applications

WB, IHC, ICC, IF, IP

Field of Research

Domain Families, Epigenetics and Nuclear Signaling, Neurogenesis, Neurology Process, Neuroscience, Transcription, Transcription Factors, Zinc Finger

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include activation or repression, in activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP) -mediated cardiac-specific gene expression (PubMed:15329343) . Binds to SMAD binding elements (SBEs) (5'- GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44713 MW

Observed Molecular Weight

68 kDa

Gene Name Synonym

Transcriptional repressor protein YY1

Subcellular Location

Nucleus matrix . Associated with the nuclear matrix.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.