Anti-YB1 YBX1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-YB1 YBX1 Rabbit Monoclonal Antibody catalog # M01054. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01054

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1; YBX1; NSEP1, YB1

Gene Name

YBX1

Gene ID

4904

UniProt

P67809

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human YB1

Clonality

Monoclonal

Clone

Clone: DDB-25

Tissue Specificity

Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Cell Type Markers, Chromatin Binding Proteins, Developmental Biology, DNA/RNA Binding, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Intracellular, Stem Cells, Surface Molecules, Transcription, Tumor Associated

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity) . Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro) . May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

35924 MW

Observed Molecular Weight

45 kDa

Gene Name Synonym

Nuclease-sensitive element-binding protein 1

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic granule . Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.