Anti-PRC1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-PRC1 Rabbit Monoclonal Antibody catalog # M00160. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00160

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Protein regulator of cytokinesis 1 ; PRC1

Gene Name

PRC1

Gene ID

9055

UniProt

O43663

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PRC1

Clonality

Monoclonal

Clone

Clone: EH-16

Tissue Specificity

Overexpressed in bladder cancer cells (PubMed:17409436) . .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Cell Biology, Cell Cycle, Cell Division, Cytokinesis

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71607 MW

Observed Molecular Weight

72 kDa

Gene Name Synonym

Protein regulator of cytokinesis 1

Subcellular Location

Nucleus . Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Midbody . Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436) . Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. .

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.