Anti-MLH1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-MLH1 Rabbit Monoclonal Antibody catalog # M00126. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00126

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1; COCA2

Gene Name

MLH1

Gene ID

4292

UniProt

P40692

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human MLH1

Clonality

Monoclonal

Clone

Clone: AED-13

Tissue Specificity

Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Cancer, DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Tumor Suppressors

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR) . DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36 kDa

Observed Molecular Weight

85 kDa

Gene Name Synonym

DNA mismatch repair protein Mlh1

Subcellular Location

Nucleus .

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.