Anti-PMS2 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-PMS2 Rabbit Monoclonal Antibody catalog # M01028-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M01028-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Mismatch repair endonuclease PMS2 ;3.1.-.-; DNA mismatch repair protein PMS2; PMS1 protein homolog 2; PMS2 ; PMSL2

Gene Name

PMS2

Gene ID

5395

UniProt

P54278

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human PMS2

Clonality

Monoclonal

Clone

Clone: AEH-16

Tissue Specificity

Ubiquitous. .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Component of the post-replicative DNA mismatch repair system (MMR) . Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

95797 MW

Observed Molecular Weight

120 kDa

Gene Name Synonym

Mismatch repair endonuclease PMS2

Subcellular Location

Nucleus .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.