Anti-KAP1 TRIM28 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-KAP1 TRIM28 Rabbit Monoclonal Antibody catalog # M00409. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00409

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Transcription intermediary factor 1-beta; TIF1-beta; E3 SUMO-protein ligase TRIM28;6.3.2.-; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28; TRIM28; KAP1, RNF96, TIF1B

Gene Name

TRIM28

Gene ID

10155

UniProt

Q13263

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human KAP1

Clonality

Monoclonal

Clone

Clone: BOE-20

Tissue Specificity

Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Death,2339, Chromatin Binding Proteins, Epigenetics and Nuclear Signaling, Invasion/Microenvironment, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ring Finger E3 Ligase, Transcription, Transcription Factors, Ubiquitin E3 Enzymes

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs) . Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me) ) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21 (CIP1) . Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

88550 MW

Observed Molecular Weight

52 kDa

Gene Name Synonym

Transcription intermediary factor 1-beta

Subcellular Location

Nucleus . Associated with centromeric heterochromatin during cell differentiation through CBX1. .

Protein Name

T-cell surface glycoprotein CD4

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.