Anti-NOX4 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-NOX4 Rabbit Monoclonal Antibody catalog # M00403. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00403

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

NADPH oxidase 4;1.6.3.-; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD (P) H-oxidase; NOX4; RENOX

Gene Name

NOX4

Gene ID

50507

UniProt

Q9NPH5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human NOX4

Clonality

Monoclonal

Clone

Clone: E-14

Tissue Specificity

Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level) . Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells. .

Applications

WB, IHC, ICC, IF, IP

Field of Research

Atherosclerosis, Cardiovascular, Cell Biology, Drug Metabolism, Heart Disease, Metabolic Signaling Pathways, Metabolism, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction, Vascular Inflammation

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

66932 MW

Observed Molecular Weight

52 kDa

Gene Name Synonym

NADPH oxidase 4

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein . Cell junction, focal adhesion . May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.

Protein Name

T-cell surface glycoprotein CD4

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.