Anti-PIM1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-PIM1 Rabbit Monoclonal Antibody catalog # M01184. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

M01184

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Serine/threonine-protein kinase pim-1;2.7.11.1; PIM1

Gene Name

PIM1

Gene ID

5292

UniProt

P11309

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human PIM1

Clonality

Monoclonal

Clone

Clone: ECB-16

Tissue Specificity

Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. .

Applications

WB, IHC, ICC, IF

Field of Research

Protein Phosphorylation, Ser/Thr Kinases, Serine/Threonine Kinases, Signal Transduction, Stem Cells

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3) . Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X (L) /BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post- translational levels. Phosphorylation of CDKN1B, induces 14-3-3- proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

45412 MW

Observed Molecular Weight

190 kDa

Gene Name Synonym

Serine/threonine-protein kinase pim-1

Subcellular Location

Isoform 2: Cytoplasm. Nucleus.

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.