Anti-ATG5/Apg5 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-ATG5/Apg5 Rabbit Monoclonal Antibody catalog # M00240. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00240

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Autophagy protein 5; APG5-like; Apoptosis-specific protein; ATG5; APG5L, ASP

Gene Name

ATG5

Gene ID

9474

UniProt

Q9H1Y0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human ATG5

Clonality

Monoclonal

Clone

Clone: GCD-1

Tissue Specificity

Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Applications

WB, IHC, ICC, IF, IP

Field of Research

Apoptosis, Associated Proteins, Cancer, Cardiovascular, Cell Biology, Cell Death, Intracellular, Metabolism, Metabolism Processes, Pathways and Processes, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Signal Transduction, Ub-Like Proteins

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12- ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32447 MW

Observed Molecular Weight

55 kDa

Gene Name Synonym

Autophagy protein 5

Subcellular Location

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity) . Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.