Anti-ARF1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-ARF1 Rabbit Monoclonal Antibody catalog # M01279-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01279-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

ADP-ribosylation factor 1; ARF1

Gene Name

ARF1

Gene ID

375

UniProt

P84077

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human ARF1

Clonality

Monoclonal

Clone

Clone: GFA-1

Tissue Specificity

Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle. .

Applications

WB, IHC, Flow Cytometry

Field of Research

ER Proteins, Protein Trafficking, Signal Transduction, Vesicle Transport

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

20697 MW

Observed Molecular Weight

70 kDa

Gene Name Synonym

ADP-ribosylation factor 1

Subcellular Location

Golgi apparatus . Cytoplasm, perinuclear region . Cell junction, synapse, synaptosome . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Membrane ; Lipid- anchor .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.