Anti-CDK4 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-CDK4 Rabbit Monoclonal Antibody catalog # M00159-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M00159-1

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Cyclin-dependent kinase 4;2.7.11.22; Cell division protein kinase 4; PSK-J3; CDK4

Gene Name

CDK4

Gene ID

1019

UniProt

P11802

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human CDK4

Clonality

Monoclonal

Clone

Clone: GIC-3

Tissue Specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level) . Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33730 MW

Observed Molecular Weight

34 kDa

Gene Name Synonym

Cyclin-dependent kinase 4

Subcellular Location

Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G (1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G (1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.