Anti-UBA1/Ube1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-UBA1/Ube1 Rabbit Monoclonal Antibody catalog # M02810. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02810

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ubiquitin-like modifier-activating enzyme 1;6.2.1.45 ; Protein A1S9; Ubiquitin-activating enzyme E1; UBA1; A1S9T, UBE1

Gene Name

UBA1

Gene ID

7317

UniProt

P22314

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human UBA1

Clonality

Monoclonal

Clone

Clone: IAC-21

Tissue Specificity

Detected in erythrocytes (at protein level) . Ubiquitous. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cell Biology, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system (PubMed:1606621, PubMed:1447181) . Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181) . Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

117849 MW

Observed Molecular Weight

118 kDa

Gene Name Synonym

Ubiquitin-like modifier-activating enzyme 1

Subcellular Location

Cytoplasm . Mitochondrion . Nucleus .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.