Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody catalog # M00053. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Specifications

Catalog number

M00053

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF; SDF-1-beta (3-72) ; SDF-1-alpha (3-67) ; CXCL12; SDF1, SDF1A, SDF1B

Gene Name

CXCL12

Gene ID

20315

UniProt

P48061

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human SDF1

Clonality

Monoclonal

Clone

Clone: IFO-3

Tissue Specificity

Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. .

Applications

WB, ICC, IF

Field of Research

Chemokines, Host-Virus Interaction, Immunology, Innate Immunity, Interspecies Interaction, Microbiology

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta (3-72) and SDF-1- alpha (3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha (3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10666 MW

Observed Molecular Weight

82 kDa

Gene Name Synonym

Stromal cell-derived factor 1

Subcellular Location

Secreted.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.