Anti-ULK1/Atg1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-ULK1/Atg1 Rabbit Monoclonal Antibody catalog # M00584. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00584

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Serine/threonine-protein kinase ULK1;2.7.11.1; Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1; ULK1; KIAA0722

Gene Name

ULK1

Gene ID

8408

UniProt

O75385

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human ULK1

Clonality

Monoclonal

Clone

Clone: ABHH-21

Tissue Specificity

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Applications

WB, IHC, ICC, IF

Field of Research

Cardiovascular, Metabolism, Mitochondrial Metabolism, Neurogenesis, Neurology Process, Neuroscience, Pathways and Processes, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

112631 MW

Observed Molecular Weight

130 kDa

Gene Name Synonym

Serine/threonine-protein kinase ULK1

Subcellular Location

Cytoplasm, cytosol . Preautophagosomal structure . Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. .

Protein Name

T-cell surface glycoprotein CD4

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.