Anti-UCHL3 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-UCHL3 Rabbit Monoclonal Antibody catalog # M05004. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M05004

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3;3.4.19.12; Ubiquitin thioesterase L3; UCHL3

Gene Name

UCHL3

Gene ID

7347

UniProt

P15374

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human UCHL3

Clonality

Monoclonal

Clone

Clone: BGE-21

Tissue Specificity

Highly expressed in heart, skeletal muscle, and testis. .

Applications

WB

Field of Research

Cell Biology, Deubiquitination, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3, and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Inly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB (+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26183 MW

Observed Molecular Weight

35 kDa

Applications Notes

WB 1:1000-1:2000

Gene Name Synonym

Ubiquitin carboxyl-terminal hydrolase isozyme L3

Subcellular Location

Cytoplasm.

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.