Anti-Nanog Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Nanog Rabbit Monoclonal Antibody catalog # M00153. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M00153

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog; NANOG

Gene Name

NANOG

Gene ID

79923

UniProt

Q9H9S0

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Nanog

Clonality

Monoclonal

Clone

Clone: EHD-14

Tissue Specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level) . Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Cancer, Cell Cycle, Cell Differentiation,2339, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Intracellular, Nuclear Signaling Pathways, Stem Cells, Surface Molecules, Transcription, Transcription Factors

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34620 MW

Observed Molecular Weight

119 kDa

Gene Name Synonym

Homeobox protein NANOG

Subcellular Location

Nucleus .

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.