Anti-c-Fos Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-c-Fos Rabbit Monoclonal Antibody catalog # M00297. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M00297

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7

Gene Name

FOS

Gene ID

2353

UniProt

P01100

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human c-Fos

Clonality

Monoclonal

Clone

Clone: IIO-6

Tissue Specificity

Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart. .

Applications

WB, IHC, IF

Field of Research

Cancer, Cancer Susceptibility, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Oncoproteins, Oncoproteins/Suppressors, Proto-Oncogenes, Transcription, Transcription Factors

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

40695 MW

Observed Molecular Weight

3 kDa

Gene Name Synonym

Proto-oncogene c-Fos

Subcellular Location

Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.