Anti-VAMP8/Endobrevin Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-VAMP8/Endobrevin Rabbit Monoclonal Antibody catalog # M02338. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M02338

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Vesicle-associated membrane protein 8 ; VAMP-8 ; Endobrevin ; EDB ; VAMP8

Gene Name

VAMP8

Gene ID

8673

UniProt

Q9BV40

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human VAMP8

Clonality

Monoclonal

Clone

Clone: AOBO-22

Tissue Specificity

Platelets. .

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Cell Type Marker, Immunology, Innate Immunity, Macrophage/Inflammation, Neuron Marker, Neuroscience, Neurotransmission, Secretory Vesicles, Synapse Marker

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

SNAREs, soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604) . Also required for dense-granule secretion in platelets (PubMed:12130530) . Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity) . Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity) . Involved in the homotypic fusion of early and late endosomes (By similarity) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

11438 MW

Observed Molecular Weight

11 kDa

Gene Name Synonym

Vesicle-associated membrane protein 8

Subcellular Location

Lysosome membrane ; Single-pass type IV membrane protein . Early endosome membrane ; Single-pass type IV membrane protein . Late endosome membrane ; Single-pass type IV membrane protein . Cell membrane ; Single-pass type IV membrane protein . Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity) . Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity) . Cycles through the apical but not through the basolateral plasma membrane (By similarity) . Apical region of acinar cells; in zymogen granule membranes (By similarity) . .

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.