Anti-Lin28 LIN28A Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-Lin28 LIN28A Rabbit Monoclonal Antibody catalog # M01966. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.

Specifications

Catalog number

M01966

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1; LIN28A; CSDD1, LIN28, ZCCHC1

Gene Name

LIN28A

Gene ID

79727

UniProt

Q9H9Z2

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Lin28

Clonality

Monoclonal

Clone

Clone: AAAG-12

Tissue Specificity

Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors. .

Applications

WB, IP, Flow Cytometry

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Translation

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, inly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity) . Suppressor of microRNA (miRNA) biogenesis, including that of let- 7, miR107, miR-143 and miR-200c. Specifically binds miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits ZCCHC11/TUT4 uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs (PubMed:19703396) (PubMed:22118463) (PubMed:22898984) . Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (By similarity) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

22743 MW

Observed Molecular Weight

26 kDa

Gene Name Synonym

Protein lin-28 homolog A

Subcellular Location

Cytoplasm . Nucleus, nucleolus. Predominantly cytoplasmic (PubMed:22118463) . Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity) . Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules. .

Protein Name

Lysosome-associated membrane glycoprotein 2 (Lamp2)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.