Anti-PRMT1 Rabbit Monoclonal Antibody

About this product

Boster Bio Anti-PRMT1 Rabbit Monoclonal Antibody catalog # M01417. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

M01417

Size / count

100 µL/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Protein arginine N-methyltransferase 1;2.1.1.319 ; Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4; PRMT1; HMT2, HRMT1L2, IR1B4

Gene Name

PRMT1

Gene ID

3276

UniProt

Q99873

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human PRMT1

Clonality

Monoclonal

Clone

Clone: AAGF-16

Tissue Specificity

Widely expressed. .

Applications

WB, IHC, ICC, IF

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Chromatin Modifying Enzymes, Co-Activators/Co-Repressors, Co-Factors, Epigenetics and Nuclear Signaling, Methylation, Nuclear Receptors, Nuclear Signaling Pathways, p53 Pathway, Transcription

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER) -mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC) -binding activity (PubMed:25284789) . Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

41516 MW

Observed Molecular Weight

40 kDa

Gene Name Synonym

Protein arginine N-methyltransferase 1

Subcellular Location

Nucleus . Nucleus, nucleoplasm . Cytoplasm, cytosol . Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity) . .

Protein Name

Glycogen synthase kinase-3 beta (gsk3b)

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.